6XUC
Structure of coproheme decarboxylase from Corynebacterium diphteriae in complex with coproheme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 170 |
| Detector technology | PIXEL |
| Collection date | 2018-11-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.021, 123.157, 77.893 |
| Unit cell angles | 90.00, 98.49, 90.00 |
Refinement procedure
| Resolution | 48.117 - 1.870 |
| R-factor | 0.1676 |
| Rwork | 0.165 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dtz |
| Data reduction software | EDNA |
| Data scaling software | EDNA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.130 | 1.661 |
| High resolution limit [Å] | 1.603 | 1.603 |
| Number of reflections | 139066 | 8944 |
| <I/σ(I)> | 6.21 | 0.4 |
| Completeness [%] | 93.6 | |
| Redundancy | 3.19 | |
| CC(1/2) | 0.996 | 0.134 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 170 | PEG 3350, MgCl2 |
