6XU5

Human myelin protein P2 mutant N2D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-19
Detector	RAYONIX MX225-HS
Wavelength(s)	0.97
Spacegroup name	P 41 21 2
Unit cell lengths	58.260, 58.260, 101.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.650
R-factor	0.1479
Rwork	0.146
R-free	0.18770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wut
RMSD bond length	0.009
RMSD bond angle	1.081
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_2247)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.700
High resolution limit [Å]	1.650	1.650
Rmeas	0.090	0.703
Number of reflections	21068	2445
<I/σ(I)>	15.2	1.5
Completeness [%]	97.4	78.9
Redundancy	4.7	2
CC(1/2)	0.998	0.625

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	36% PEG 6000, 0.1 M citrate pH 5.5