6XTZ

Structure of Dally-like protein in complex with O-palmitoleoyl serine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-27
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97954
Spacegroup name	P 21 21 21
Unit cell lengths	63.691, 77.380, 118.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.140 - 2.210
R-factor	0.2281
Rwork	0.227
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3odn
RMSD bond length	0.002
RMSD bond angle	0.369
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (dev_3488)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.140	2.250
High resolution limit [Å]	2.210	2.210
Number of reflections	30164	1459
<I/σ(I)>	8
Completeness [%]	100.0
Redundancy	13.1
CC(1/2)	0.998	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	16.25% (w/v) PEG4000, 0.1 M MMT pH 9