6XTU

FULL-LENGTH LTTR LYSG FROM CORYNEBACTERIUM GLUTAMICUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-06-10
Detector	PSI PILATUS 6M
Wavelength(s)	1.044
Spacegroup name	P 41 21 2
Unit cell lengths	124.980, 124.980, 111.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.940 - 2.520
R-factor	0.1964
Rwork	0.195
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3isp
RMSD bond length	0.006
RMSD bond angle	0.817
Data reduction software	XDS (November 3, 2014q)
Data scaling software	XSCALE (November 3, 2014 BUILT=20141118)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.940	2.610
High resolution limit [Å]	2.520	2.520
Rmerge	0.064	1.206
Rmeas	0.067	1.273
Number of reflections	30335	2992
<I/σ(I)>	25.02	2.17
Completeness [%]	99.9	100
Redundancy	9.6	9.9
CC(1/2)	1.000	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	17.5% PEG4000, 0.1 M AMMONIUMSULFATE, 0.1 M TRIS-HCL PH 8.0