6XTJ

The high resolution structure of the FERM domain of human FERMT2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 32 2 1
Unit cell lengths	145.172, 145.172, 59.633
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	72.700 - 1.600
Rwork	0.181
R-free	0.20370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xpy
RMSD bond length	0.008
RMSD bond angle	1.428
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.700	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.277	5.732
Rmeas	0.280	5.965
Rpim	0.071	1.638
Number of reflections	95151	4659
<I/σ(I)>	11	1
Completeness [%]	100.0	99.7
Redundancy	31.1	25.4
CC(1/2)	0.999	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1M HEPES pH 7.0, 1.4M sodium citrate tribasic