6XSI
Crystal structure of Staphylococcal nuclease variant Delta+PHS V104T at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2018-03-28 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.920, 60.620, 38.320 |
| Unit cell angles | 90.00, 93.60, 90.00 |
Refinement procedure
| Resolution | 24.800 - 2.000 |
| R-factor | 0.2371 |
| Rwork | 0.235 |
| R-free | 0.27050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.425 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.020 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.022 | 0.204 |
| Number of reflections | 9632 | 965 |
| <I/σ(I)> | 38.13 | 4.53 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, THP |
