6XSF
Crystal structure of Staphylococcal nuclease variant Delta+PHS T41V at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2016-09-13 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.220, 60.460, 38.240 |
Unit cell angles | 90.00, 93.36, 90.00 |
Refinement procedure
Resolution | 24.880 - 1.600 |
R-factor | 0.1904 |
Rwork | 0.189 |
R-free | 0.22740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.570 |
Data reduction software | CrysalisPro |
Data scaling software | CrysalisPro |
Phasing software | PHASER (2.7.17) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.170 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.015 | 0.102 |
Number of reflections | 18805 | 1885 |
<I/σ(I)> | 58.61 | 8.82 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.1 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 35% MPD, 25 mM potassium phosphate, calcium chloride, THP |