6XSB
Crystal structure of Staphylococcal nuclease variant Delta+PHS A132S at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2018-04-04 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.110, 60.600, 38.090 |
| Unit cell angles | 90.00, 93.26, 90.00 |
Refinement procedure
| Resolution | 23.710 - 1.950 |
| R-factor | 0.2141 |
| Rwork | 0.212 |
| R-free | 0.25860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.661 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.030 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.018 | 0.133 |
| Number of reflections | 10374 | 1041 |
| <I/σ(I)> | 55.48 | 6.55 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.4 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 25% MPD, 25 mM potassium phosphate, calcium chloride, THP |
