6XS4

Crystal structure of glycyl radical enzyme ECL_02896 from Enterobacter cloacae subsp. cloacae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	283.101, 60.974, 118.951
Unit cell angles	90.00, 107.65, 90.00

Refinement procedure

Resolution	39.720 - 2.330
R-factor	0.18
Rwork	0.179
R-free	0.20330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mtj
RMSD bond length	0.003
RMSD bond angle	0.564
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.724	2.380
High resolution limit [Å]	2.330	2.340
Rpim	0.045	0.192
Number of reflections	78987	3022
<I/σ(I)>	9.25
Completeness [%]	95.4
Redundancy	3.5
CC(1/2)		0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	298	15% PEG3350, 0.1M Succinic Acid pH 7