6XRJ
Crystal structure of the disulfide linked DH717.1 Fab dimer, derived from a macaque HIV-1 vaccine-induced Env glycan-reactive neutralizing antibody B cell lineage
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 193.15 |
Detector technology | PIXEL |
Collection date | 2018-03-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 201.587, 201.587, 154.327 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.710 - 3.150 |
R-factor | 0.2464 |
Rwork | 0.245 |
R-free | 0.28220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ukn |
RMSD bond length | 0.012 |
RMSD bond angle | 2.034 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.710 | 3.260 |
High resolution limit [Å] | 3.147 | 3.147 |
Rmerge | 0.166 | |
Number of reflections | 55412 | 5393 |
<I/σ(I)> | 13.4 | 1.3 |
Completeness [%] | 99.7 | 98.94 |
Redundancy | 9 | 9.6 |
CC(1/2) | 0.992 | 0.525 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 295.15 | 0.1M Citric acid pH3.5, 14% PEG 1000 |