6XR4
Integrative in situ structure of Parkinsons disease-linked human LRRK2
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Quantum SE |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 14 |
| Software | RELION,SerialEM,IMP |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
