6XR0
Crystal Structure of Human Melanotransferrin in complex with SC57.32 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 297 |
| Detector technology | PIXEL |
| Collection date | 2018-07-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.829, 136.910, 111.135 |
| Unit cell angles | 90.00, 91.46, 90.00 |
Refinement procedure
| Resolution | 111.100 - 3.064 |
| R-factor | 0.2033 |
| Rwork | 0.202 |
| R-free | 0.22490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b1x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7 (19-MAR-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.100 | 3.117 |
| High resolution limit [Å] | 3.060 | 3.064 |
| Rpim | 0.084 | 0.621 |
| Number of reflections | 32506 | 1623 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 100 mM sodium acetate, pH 4.6 and 30% PEG300 |
