6XQW
Crystal Structure of MaliM03 Fab in complex with Pfmsp1-19
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.737, 69.451, 65.834 |
Unit cell angles | 90.00, 108.24, 90.00 |
Refinement procedure
Resolution | 47.016 - 2.991 |
R-factor | 0.2545 |
Rwork | 0.252 |
R-free | 0.30920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ob1 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.659 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.016 | 3.050 |
High resolution limit [Å] | 2.990 | 3.000 |
Number of reflections | 10456 | 498 |
<I/σ(I)> | 10.7 | |
Completeness [%] | 99.9 | |
Redundancy | 3.3 | |
CC(1/2) | 0.990 | 0.890 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20% w/v PEG 8K, 40% v/v PEG 400, 0.5M NaCl and 0.1 M acetate buffer pH 5.5 |