6XQW
Crystal Structure of MaliM03 Fab in complex with Pfmsp1-19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.737, 69.451, 65.834 |
| Unit cell angles | 90.00, 108.24, 90.00 |
Refinement procedure
| Resolution | 47.016 - 2.991 |
| R-factor | 0.2545 |
| Rwork | 0.252 |
| R-free | 0.30920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ob1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.659 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.016 | 3.050 |
| High resolution limit [Å] | 2.990 | 3.000 |
| Number of reflections | 10456 | 498 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.990 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20% w/v PEG 8K, 40% v/v PEG 400, 0.5M NaCl and 0.1 M acetate buffer pH 5.5 |
