6XQK
Crystal structure of the D/D domain of PKA from S. cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 108 |
Detector technology | IMAGE PLATE |
Collection date | 2013-03-27 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54179 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.824, 52.134, 68.832 |
Unit cell angles | 90.00, 109.70, 90.00 |
Refinement procedure
Resolution | 64.800 - 2.560 |
R-factor | 0.217 |
Rwork | 0.214 |
R-free | 0.27000 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.008 |
RMSD bond angle | 0.850 |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.800 | 2.690 |
High resolution limit [Å] | 2.557 | 2.560 |
Rmerge | 0.125 | 0.440 |
Number of reflections | 12426 | 1777 |
<I/σ(I)> | 12 | |
Completeness [%] | 99.7 | 99.1 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 24% w/v PEG4000, 0.1 M Tris, pH 8.5, 0.2 M magnesium chloride |