6XQ6
Receptor for Advanced Glycation End Products VC1 domain in complex with Fragment 11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-08 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 62 |
Unit cell lengths | 101.376, 101.376, 104.025 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.440 - 1.900 |
R-factor | 0.1963 |
Rwork | 0.195 |
R-free | 0.22060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lp4 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.374 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.960 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmeas | 0.076 | 0.286 |
Rpim | 0.026 | 0.100 |
Number of reflections | 94132 | 9443 |
<I/σ(I)> | 14.4 | |
Completeness [%] | 99.6 | |
Redundancy | 4.1 | 7.72 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2.5 M NaOAc (pH 7.5) |