6XPQ

Human antibody D1 H1-17/H3-14 in complex with the influenza hemagglutinin head domain of A/Texas/50/2012(H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-03-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	C 2 2 21
Unit cell lengths	115.360, 162.800, 202.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.105 - 4.200
R-factor	0.2744
Rwork	0.273
R-free	0.30650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5W08 & 6E4X
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.105	4.350
High resolution limit [Å]	4.200	4.200
Rmerge	0.107	0.909
Rmeas	0.124	1.047
Rpim	0.062	0.511
Number of reflections	13796	1386
<I/σ(I)>	7.62	1.19
Completeness [%]	96.6	98.16
Redundancy	3.7	3.8
CC(1/2)	0.997	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.5 M lithium chloride, 1.0 M sodium citrate