6XPO
Influenza hemagglutinin A/Bangkok/01/1979(H3N2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-03-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 200.660, 186.180, 107.400 |
| Unit cell angles | 90.00, 110.13, 90.00 |
Refinement procedure
| Resolution | 48.658 - 3.000 |
| R-factor | 0.2277 |
| Rwork | 0.226 |
| R-free | 0.26220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mxu |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.660 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.162 | 1.101 |
| Rmeas | 0.187 | |
| Rpim | 0.092 | 0.614 |
| Number of reflections | 71349 | 7168 |
| <I/σ(I)> | 7.88 | 1.2 |
| Completeness [%] | 96.3 | 97.12 |
| Redundancy | 3.9 | 4.1 |
| CC(1/2) | 0.993 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 5% (w/v) PEG 400, 0.5M Sodium citrate, pH7.0 |
