6XPH

CutR dimer with domain swap

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979200
Spacegroup name	P 43 3 2
Unit cell lengths	110.290, 110.290, 110.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.541 - 1.800
Rwork	0.176
R-free	0.21020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4axi
RMSD bond length	0.014
RMSD bond angle	1.840
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	77.541	77.541	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.079	0.030	1.391
Rmeas	0.079	0.030	1.401
Number of reflections	21478	309	1557
<I/σ(I)>	49.24	132.89	4.71
Completeness [%]	100.0	100	100
Redundancy	75.744	58.1	75.197
CC(1/2)	1.000	1.000	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M Potassium thiocyanate, 30% w/v PEG 2000