6XOD

Crystal structure of the PEX4-PEX22 protein complex from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	57.389, 100.484, 112.092
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.830 - 2.010
R-factor	0.1958
Rwork	0.194
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nkz
RMSD bond length	0.003
RMSD bond angle	0.565
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.046	56.046	2.042
High resolution limit [Å]	2.008	5.448	2.008
Rmerge	0.096	0.056	1.559
Rmeas	0.104	0.062	1.711
Rpim	0.041	0.025	0.696
Number of reflections	22014	1201	1079
<I/σ(I)>	10.5	27.7	1.1
Completeness [%]	99.9	99.7	100
Redundancy	6.6	6.1	6
CC(1/2)	0.998	0.998	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	100 mM NaCl, 100 mM Bis-Tris Propane, 25% (w/v) PEG 1,500