6XND
Avidin-Biotin-Phenol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2020-02-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.526, 79.254, 74.683 |
Unit cell angles | 90.00, 105.46, 90.00 |
Refinement procedure
Resolution | 44.843 - 1.580 |
R-factor | 0.2351 |
Rwork | 0.234 |
R-free | 0.27180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2avi |
RMSD bond length | 0.012 |
RMSD bond angle | 1.525 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.843 | 1.640 |
High resolution limit [Å] | 1.580 | 1.580 |
Number of reflections | 71463 | 6991 |
<I/σ(I)> | 10.23 | |
Completeness [%] | 99.9 | |
Redundancy | 6.4 | |
CC(1/2) | 0.999 | 0.462 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 300 | 0.1 M Tris, pH 7.0, and 25% (w/v) PEG 1500 |