6XMM
Human aldolase A I98S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 162.213, 162.213, 167.852 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.060 - 2.110 |
| R-factor | 0.2366 |
| Rwork | 0.234 |
| R-free | 0.29470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ald |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.695 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 140.500 | 2.420 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rpim | 0.068 | 0.738 |
| Number of reflections | 39448 | 1972 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 88.4 | |
| Redundancy | 18.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 10% (w/v) PEG 8000, 15% (v/v) glycerol, 0.04 M potassium phosphate, pH 6.0 |
