6XMI
Structure of Fab4 bound to P22 TerL(1-33)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-21 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.180, 86.395, 86.151 |
Unit cell angles | 90.00, 97.72, 90.00 |
Refinement procedure
Resolution | 14.985 - 1.510 |
R-factor | 0.1677 |
Rwork | 0.167 |
R-free | 0.20320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6VHM |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.16) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.560 |
High resolution limit [Å] | 1.510 | 1.510 |
Rpim | 0.042 | 0.573 |
Number of reflections | 161937 | 15816 |
<I/σ(I)> | 32.7 | 1.9 |
Completeness [%] | 96.0 | 94.3 |
Redundancy | 2.9 | 2.7 |
CC(1/2) | 0.995 | 0.456 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 0.2 M Succinic acid pH 7.0 and 20% w/v Polyethylene glycol 3,350 |