6XMH
Human aldolase A wild type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 161.885, 161.885, 168.128 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.850 - 1.950 |
| R-factor | 0.241 |
| Rwork | 0.239 |
| R-free | 0.28610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ald |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.543 |
| Refinement software | REFMAC (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 140.200 | 2.200 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rpim | 0.046 | 0.356 |
| Number of reflections | 549062 | 2830 |
| <I/σ(I)> | 10.7 | 2.2 |
| Completeness [%] | 93.8 | |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.75 | 277 | 10% (w/v) PEG 8,000, 18% (v/v) glycerol, 0.04 M potassium phosphate, pH 5.75 |
