6XMD
SM Protein Vps45 in Complex with Qa SNARE Tlg2 (1-310)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97935 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 58.380, 89.434, 209.137 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.510 - 3.900 |
R-factor | 0.1932 |
Rwork | 0.191 |
R-free | 0.24250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xjl |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHASER |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.510 | 29.510 | 4.340 |
High resolution limit [Å] | 3.880 | 8.670 | 3.880 |
Rmerge | 0.246 | 0.088 | 2.149 |
Rmeas | 0.256 | 0.092 | 2.234 |
Rpim | 0.071 | 0.027 | 0.603 |
Total number of observations | 138267 | 12417 | 38856 |
Number of reflections | 10653 | 1040 | 2891 |
<I/σ(I)> | 6.9 | 27.5 | 1.3 |
Completeness [%] | 99.3 | 97.8 | 98.1 |
Redundancy | 13 | 11.9 | 13.4 |
CC(1/2) | 0.993 | 0.990 | 0.649 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4 mg/ml protein in well buffer consisting of 0.125 M potassium citrate, 15 % (w/v) PEG 3350, 15% (v/v) glycerol, and 1 mM DTT |