6XM6

Crystal structure of cobalt-bound LSD4 from Sphingobium sp. strain SYK-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97857
Spacegroup name	I 2 2 2
Unit cell lengths	85.480, 112.180, 114.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.847 - 1.450
R-factor	0.1399
Rwork	0.139
R-free	0.15890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ojr
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.847	43.847	1.550
High resolution limit [Å]	1.450	5.000	1.450
Rmerge	0.051	0.041	0.385
Rmeas	0.061	0.048	0.466
Total number of observations	628528
Number of reflections	188761	4602	34159
<I/σ(I)>	14.02	25.69	3.04
Completeness [%]	99.9	99.7	99.6
Redundancy	3.33	3.404	3.153
CC(1/2)	0.996	0.996	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.3 M magnesium acetate, 29% PEG3350