6XM1
SM Protein Vps45 in Complex with Qa SNARE Tlg2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.727, 180.057, 202.086 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.680 - 2.800 |
R-factor | 0.1972 |
Rwork | 0.194 |
R-free | 0.24820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xjl |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.680 | 29.680 | 2.890 |
High resolution limit [Å] | 2.800 | 11.540 | 2.800 |
Rmerge | 0.138 | 0.030 | 1.880 |
Rmeas | 0.144 | 0.032 | 1.961 |
Rpim | 0.039 | 0.009 | 0.549 |
Total number of observations | 10199 | 55507 | |
Number of reflections | 53946 | 841 | 4521 |
<I/σ(I)> | 13.6 | 47.9 | 1.4 |
Completeness [%] | 99.8 | 94.6 | 98.9 |
Redundancy | 13.4 | 12.1 | 12.3 |
CC(1/2) | 0.999 | 1.000 | 0.731 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2.5 mg/ml protein in well buffer, consisting of 0.1 M HEPES pH 8.0, 0.1 M NaCl, 11% (v/v) 2-propanol, and 5 mM TCEP |