6XL7

Structure of a mosquito complement inhibitor from Anopheles freeborni

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	85.980, 85.980, 46.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.430 - 1.420
R-factor	0.1798
Rwork	0.179
R-free	0.19320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	albicin
RMSD bond length	0.005
RMSD bond angle	0.855
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.430	31.430	1.460
High resolution limit [Å]	1.420	6.350	1.420
Rmerge	0.051	0.058	0.222
Rmeas	0.054	0.062	0.250
Total number of observations	425154
Number of reflections	32895	442	2145
<I/σ(I)>	31.38	48.06	5.34
Completeness [%]	99.2	99.8	88.6
Redundancy	12.925	10.735	4.501
CC(1/2)	0.999	0.997	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	1.8 M ammonium sulfate, 0.1 M Tris HCl