6XKN
Class III PreQ1 riboswitch mutant A52G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.6279 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 80.137, 80.137, 281.457 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.710 - 2.730 |
R-factor | 0.2368 |
Rwork | 0.236 |
R-free | 0.24660 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4rzd |
RMSD bond length | 0.001 |
RMSD bond angle | 0.468 |
Data reduction software | HKL-2000 (v708c) |
Data scaling software | HKL-2000 (v708c) |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.710 | 2.830 |
High resolution limit [Å] | 2.730 | 2.730 |
Rpim | 0.035 | 0.357 |
Number of reflections | 15078 | 1473 |
<I/σ(I)> | 20 | 1.7 |
Completeness [%] | 97.3 | 98.8 |
Redundancy | 8.4 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 85% Tacsimate pH 7.0, 10 mM magnesium acetate, 6 mM cobalt haxammine, 1 mM spermine |