6XKB
Crystal structure of SR-related and CTD-associated factor 4(SCAF4-CID)with peptide S2,S5p-CTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.718, 46.923, 139.250 |
Unit cell angles | 90.00, 92.30, 90.00 |
Refinement procedure
Resolution | 44.500 - 1.600 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.21040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d9i |
RMSD bond length | 0.007 |
RMSD bond angle | 1.452 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
Rmerge | 0.058 | 0.034 | 0.756 |
Rmeas | 0.064 | 0.037 | 0.829 |
Rpim | 0.025 | 0.015 | 0.334 |
Total number of observations | 715061 | ||
Number of reflections | 114559 | 5989 | 5678 |
<I/σ(I)> | 7.6 | ||
Completeness [%] | 94.5 | 94.7 | 94.8 |
Redundancy | 6.2 | 6 | 5.8 |
CC(1/2) | 0.999 | 0.813 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 25% PEG 3350, 0.2 M NH4OAc, 0.1 M HEPES pH 7.5 |