6XK1
Biuret Hydrolase (BiuH) from Rhodococcus sp. Mel C169S Apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.991840 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.100, 104.400, 135.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.837 - 1.700 |
| Rwork | 0.184 |
| R-free | 0.20790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xjm |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.647 |
| Data reduction software | XDS (BUILT=20200131) |
| Data scaling software | XSCALE (BUILT=20200131) |
| Phasing software | MOLREP (11.0) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.660 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.077 | 0.501 |
| Number of reflections | 186186 | 29499 |
| <I/σ(I)> | 11.55 | 2.96 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.85 | 3.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 1uL 10mg/mL protein + 1uL 18% w/v PEG3350, 0.2M MgCl2, 1mM triuret pH 5.5 |
