6XJW
Crystal structure of a self-alkylating ribozyme - alkylated form without biotin moiety
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-03-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.916, 99.639, 90.544 |
Unit cell angles | 90.00, 97.10, 90.00 |
Refinement procedure
Resolution | 89.850 - 1.918 |
R-factor | 0.1884 |
Rwork | 0.188 |
R-free | 0.22710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mwn |
RMSD bond length | 0.007 |
RMSD bond angle | 1.036 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.640 | 1.950 |
High resolution limit [Å] | 1.918 | 1.920 |
Rmerge | 0.086 | 1.901 |
Rmeas | 0.101 | 2.239 |
Rpim | 0.054 | 1.175 |
Number of reflections | 111123 | 5188 |
<I/σ(I)> | 8.7 | 0.6 |
Completeness [%] | 98.3 | 92.8 |
Redundancy | 3.5 | 3.5 |
CC(1/2) | 0.998 | 0.247 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | 0.1 M ammonium fluoride, 10% PEG3350, pH 6.2 |