6XJQ
Crystal structure of a self-alkylating ribozyme - alkylated form with biotinylated epoxide substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-03-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.962, 99.832, 90.636 |
Unit cell angles | 90.00, 97.20, 90.00 |
Refinement procedure
Resolution | 83.300 - 1.708 |
R-factor | 0.1894 |
Rwork | 0.189 |
R-free | 0.21320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mwn |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.300 | 1.740 |
High resolution limit [Å] | 1.708 | 1.710 |
Rmerge | 0.056 | 1.848 |
Rmeas | 0.066 | 2.187 |
Rpim | 0.035 | 1.160 |
Number of reflections | 159200 | 7389 |
<I/σ(I)> | 11.2 | 0.6 |
Completeness [%] | 99.3 | 93.3 |
Redundancy | 3.4 | 3.4 |
CC(1/2) | 0.999 | 0.235 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | 0.1 M ammonium fluoride, 10% PEG3350, pH 6.2 |