6XJN
Human atlastin-1 (residues 1-439) bound to GDP-Mg2+ exhibits an ordered amino terminus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-17 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9767 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 54.170, 138.860, 202.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.860 - 2.200 |
R-factor | 0.2021 |
Rwork | 0.201 |
R-free | 0.23690 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.898 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.735 | |
Rmeas | 0.078 | |
Number of reflections | 39278 | 5640 |
<I/σ(I)> | 16.2 | |
Completeness [%] | 100.0 | |
Redundancy | 8.9 | |
CC(1/2) | 0.998 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 3.5 M sodium formate pH 7, crystals transferred to cryo solution supplemented with 25% glycerol |