6XJF

X-ray crystal structure of Pyrococcus furiosus general transcription factor TFE-alpha (SeMet labeled protein)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	76.199, 115.610, 124.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.440 - 3.200
R-factor	0.2421
Rwork	0.237
R-free	0.30370
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.869
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.130	1.488
Number of reflections	20562	38448
<I/σ(I)>	19.818
Completeness [%]	99.8	99.8
Redundancy	14	11.5
CC(1/2)	0.993	0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	10 % PEG 8000, 0.2 M MgCl2, and 0.1 M Tris-HCl (pH 7)