6XIA

REFINEMENT OF GLUCOSE ISOMERASE FROM STREPTOMYCES ALBUS AT 1.65 ANGSTROMS WITH DATA FROM AN IMAGING PLATE

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	93.900, 99.700, 102.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.650
R-factor	0.141
RMSD bond length	0.012
RMSD bond angle	0.034
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.650 *	0.165 *
Rmerge	0.126 *	0.126 *
Total number of observations	224189 *
Number of reflections	54575 *
Completeness [%]	94.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5 *	277 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme	20 (mg/ml)
2	1	1	HEPES	0.005 (M)
3	1	1	ammonium sulphate	1 (M)