6XH1

Co-crystal structure of HIV-1 TAR RNA in complex with lab-evolved RRM TBP6.7 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.980
Spacegroup name	P 43 21 2
Unit cell lengths	40.226, 40.226, 293.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.850 - 2.601
R-factor	0.2185
Rwork	0.216
R-free	0.25490
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	Aimless (0.74)
Phasing software	PHENIX (1.15.2_3472)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.850	2.720
High resolution limit [Å]	2.600	2.600
Rpim	0.065	0.550
Number of reflections	7660	944
<I/σ(I)>	7.7	2.4
Completeness [%]	96.5	97.7
Redundancy	7.9	7.9
CC(1/2)	0.986	0.403

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	293.15	0.2 M Ammonium Nitrate, 20% (w/v) PEG-3350