6XGX

ISCth4 transposase, strand transfer complex 1, STC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2019-05-13
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	114.570, 114.570, 232.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.200 - 3.500
R-factor	0.2631
Rwork	0.261
R-free	0.29740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xg8
RMSD bond length	0.003
RMSD bond angle	0.572
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.200	3.590
High resolution limit [Å]	3.500	3.500
Rmerge	0.165	1.856
Rmeas	0.169
Number of reflections	22945	1681
<I/σ(I)>	14.54	2.15
Completeness [%]	99.8	100
Redundancy	21.55	22.08
CC(1/2)	0.999	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293.15	sodium acetate, ammonium sulfate, PEG4000, zinc chloride