6XGX
ISCth4 transposase, strand transfer complex 1, STC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2019-05-13 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 114.570, 114.570, 232.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.200 - 3.500 |
| R-factor | 0.2631 |
| Rwork | 0.261 |
| R-free | 0.29740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xg8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.572 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.200 | 3.590 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.165 | 1.856 |
| Rmeas | 0.169 | |
| Number of reflections | 22945 | 1681 |
| <I/σ(I)> | 14.54 | 2.15 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 21.55 | 22.08 |
| CC(1/2) | 0.999 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293.15 | sodium acetate, ammonium sulfate, PEG4000, zinc chloride |
