6XGP

YSD1_17 major capsid protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	54.396, 91.994, 89.032
Unit cell angles	90.00, 102.42, 90.00

Refinement procedure

Resolution	30.830 - 2.600
R-factor	0.2211
Rwork	0.219
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bqw
RMSD bond length	0.010
RMSD bond angle	1.140
Data reduction software	XDS (20151015)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.6.0)
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.010	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.106	0.734
Rmeas	0.149	1.038
Rpim	0.106	0.734
Number of reflections	26350	2620
<I/σ(I)>	8	1.4
Completeness [%]	99.6	99.6
Redundancy	2	2
CC(1/2)	0.983	0.438

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	4 % PEG3350, 20 % glycerol, 0.2 M NaBr and 0.1 M MOPS-HEPES buffer (pH 7.5)