6XFX
Self-assembly of a 3D DNA crystal lattice (4x5 junction version) containing the J26 immobile Holliday junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 67.634, 67.634, 60.662 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.136 - 3.129 |
| R-factor | 0.2292 |
| Rwork | 0.227 |
| R-free | 0.27070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6x8c |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.724 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
| Rmerge | 0.077 | 0.040 | 0.326 |
| Rmeas | 0.080 | 0.041 | 0.338 |
| Rpim | 0.020 | 0.011 | 0.083 |
| Number of reflections | 2902 | 156 | 95 |
| <I/σ(I)> | 6.4 | ||
| Completeness [%] | 96.3 | 88.1 | 66.9 |
| Redundancy | 17 | 15.6 | 13.1 |
| CC(1/2) | 1.000 | 0.999 | 0.991 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.0 with 10 mM MgCl2, 2.5 mM spermine, 5 mM CaCl2, and 10% Isopropanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |
