6XFW
Self-assembly of a 3D DNA crystal lattice (4x5 junction version) containing the J24 immobile Holliday junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.458, 68.458, 60.214 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.246 - 3.053 |
| R-factor | 0.2444 |
| Rwork | 0.243 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6x8c |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.667 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.100 |
| High resolution limit [Å] | 3.050 | 8.270 | 3.050 |
| Rmerge | 0.054 | 0.030 | 0.920 |
| Rmeas | 0.056 | 0.032 | 0.950 |
| Rpim | 0.013 | 0.009 | 0.232 |
| Number of reflections | 3313 | 189 | 164 |
| <I/σ(I)> | 7.3 | ||
| Completeness [%] | 99.9 | 99 | 99.4 |
| Redundancy | 18.7 | 16.4 | 15.9 |
| CC(1/2) | 0.977 | 1.000 | 0.905 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 7.0 with 30 mM MgCl2, 2.5 mM spermine, and 5% PEG 400 was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |
