6XFU
PmtCD peptide exporter basket domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 40.462, 45.375, 73.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.460 - 1.400 |
R-factor | 0.1625 |
Rwork | 0.161 |
R-free | 0.19370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6u2d |
RMSD bond length | 0.014 |
RMSD bond angle | 1.871 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.370 | 45.370 | 1.420 |
High resolution limit [Å] | 1.400 | 7.670 | 1.400 |
Rmerge | 0.098 | 0.069 | 0.653 |
Rmeas | 0.103 | 0.073 | 0.717 |
Rpim | 0.029 | 0.022 | 0.287 |
Total number of observations | 314139 | 2126 | 6889 |
Number of reflections | 27091 | 213 | 1152 |
<I/σ(I)> | 14.7 | 27.7 | 2.1 |
Completeness [%] | 98.5 | 99.7 | 85.4 |
Redundancy | 11.6 | 10 | 6 |
CC(1/2) | 0.998 | 0.998 | 0.759 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 0.2 M sodium thiocyanate and 20% Poly Ethylene Glycol (PEG) 3350 |