6XFL

Structural characterization of the type III secretion system pilotin-secretin complex InvH-InvG by NMR spectroscopy

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE		500
2	Bruker	AVANCE		850

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	1	3D hbCBcgcdHD
11	1	1	3D HBHA(CBCACO)NH
12	1	1	2D 1H-13C HSQC
13	1	2	3D 1H-15N NOESY
14	1	2	3D 1H-13C NOESY aromatic
15	1	2	3D HNCACB
16	1	2	3D HNCO
17	1	2	2D 1H-15N HSQC
18	1	1	3D 1H-15N NOESY filtered-edited
19	1	1	3D 1H-13C NOESY filtered-edited
2	1	1	3D HNCACB
20	1	1	3D 1H-15N NOESY
21	1	1	3D 1H-13C NOESY
22	1	2	3D 1H-13C NOESY aliphatic
23	1	2	3D hbCBcgcCH
24	1	2	3D hbCBcgcdceHE
25	1	2	3D hbCBcgcdHD
26	1	2	3D HBHA(CBCACO)NH
27	1	2	2D 1H-13C HSQC
28	1	2	3D HCC(CO)NH-TOCSY
29	1	2	3D HCCH-TOCSY
3	1	1	3D CBCA(CO)NH
30	1	2	3D HN(CA)CO
31	1	2	3D CBCA(CO)NH
32	1	2	3D 1H-15N NOESY filtered-edited
33	1	2	3D 1H-13C NOESY filtered-edited
4	1	1	3D HN(CA)CO
5	1	1	3D HCCH-TOCSY
6	1	1	3D HNCO
7	1	1	3D HCC(CO)NH-TOCSY
8	1	1	3D hbCBcgcCH
9	1	1	3D hbCBcgcdceHE

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.5	1	298

Conformers

Conformers Calculated Total Number	100
Conformers Submitted Total Number	20