6XFK
Crystal structure of the type III secretion system pilotin-secretin complex InvH-InvG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 36.313, 36.313, 221.602 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.930 - 1.850 |
R-factor | 0.2189 |
Rwork | 0.217 |
R-free | 0.24650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xfj |
RMSD bond length | 0.014 |
RMSD bond angle | 1.766 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.930 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 8226 | 788 |
<I/σ(I)> | 16.8 | |
Completeness [%] | 99.5 | |
Redundancy | 16.5 | |
CC(1/2) | 1.000 | 0.908 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 0.5 M lithium sulfate, 8.3% PEG 6000, 100 mM sodium acetate |