6XEK
Self-assembly of a 3D DNA crystal lattice (4x5 junction version) containing the J14 immobile Holliday junction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.92 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 68.908, 68.908, 62.094 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.850 |
R-factor | 0.2422 |
Rwork | 0.241 |
R-free | 0.26270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6x8c |
RMSD bond length | 0.011 |
RMSD bond angle | 1.843 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.680 | 50.000 | 2.900 |
High resolution limit [Å] | 2.850 | 7.730 | 2.850 |
Rmerge | 0.076 | 0.072 | 0.746 |
Rmeas | 0.079 | 0.075 | 0.766 |
Rpim | 0.019 | 0.020 | 0.171 |
Number of reflections | 4213 | 231 | 212 |
<I/σ(I)> | 8.7 | ||
Completeness [%] | 99.8 | 95.9 | 100 |
Redundancy | 19.1 | 16.6 | 19.4 |
CC(1/2) | 0.917 | 0.942 | 0.916 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.0 with 10 mM MgCl2, 2.5 mM spermine, 5 mM CaCl2, and 10% Isopropanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |