6XCJ
Crystal Structure of DH650 Fab from a Rhesus Macaque in Complex with HIV-1 gp120 Core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2017-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.999919 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 138.520, 122.100, 53.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.930 - 2.800 |
| R-factor | 0.2296 |
| Rwork | 0.227 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lst |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.900 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 23016 | 3659 |
| <I/σ(I)> | 13.99 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5.5 | |
| CC(1/2) | 0.998 | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG 8K, 100mM Tris pH 8, 500mM NaCl |
