6XCJ

Crystal Structure of DH650 Fab from a Rhesus Macaque in Complex with HIV-1 gp120 Core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	293
Detector technology	CCD
Collection date	2017-12-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.999919
Spacegroup name	P 21 21 2
Unit cell lengths	138.520, 122.100, 53.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.930 - 2.800
R-factor	0.2296
Rwork	0.227
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lst
RMSD bond length	0.008
RMSD bond angle	1.020
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.900	2.970
High resolution limit [Å]	2.800	2.800
Number of reflections	23016	3659
<I/σ(I)>	13.99
Completeness [%]	99.8
Redundancy	5.5
CC(1/2)	0.998	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	20% PEG 8K, 100mM Tris pH 8, 500mM NaCl