6XBC
Crystal structure of Streptomyces sviceus SsDesB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.046, 151.174, 141.401 |
Unit cell angles | 90.00, 91.58, 90.00 |
Refinement procedure
Resolution | 39.119 - 2.863 |
R-factor | 0.202 |
Rwork | 0.199 |
R-free | 0.25820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5o8r |
Data reduction software | HKL-2000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.860 | 2.860 |
Rmerge | 0.043 | 0.355 |
Rmeas | 0.079 | 0.537 |
Rpim | 0.042 | 0.288 |
Number of reflections | 78957 | 3917 |
<I/σ(I)> | 2.2 | 2.2 |
Completeness [%] | 98.7 | |
Redundancy | 3.2 | |
CC(1/2) | 0.996 | 0.860 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M HEPES pH 7.5, 0.2M NaCl, 20% w/v PEG3350, 10 mM taurine |