6XB4
Structure of PrGV poxin in post-reactive state with Gp[2'-5']Ap[3']
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.470, 58.140, 100.698 |
| Unit cell angles | 90.00, 108.55, 90.00 |
Refinement procedure
| Resolution | 38.640 - 1.900 |
| R-factor | 0.1833 |
| Rwork | 0.183 |
| R-free | 0.20680 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.568 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.640 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.057 | 0.934 |
| Number of reflections | 66856 | 4149 |
| <I/σ(I)> | 9.7 | 1.4 |
| Completeness [%] | 99.4 | |
| Redundancy | 7 | |
| CC(1/2) | 0.997 | 0.472 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 200 mM magnesium chloride, 100 mM Tris-Cl, 21-25% PEG-3350 |
