6X9X

Crystal structure of Fab fragment of Anti-HCV E2 antibody (HC84.26)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	82.535, 113.699, 122.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.900 - 1.800
R-factor	0.17993
Rwork	0.178
R-free	0.20966
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5esa
RMSD bond length	0.012
RMSD bond angle	1.472
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.053	0.649
Rmeas	0.059	0.728
Rpim	0.025	0.326
Number of reflections	53599	5293
<I/σ(I)>	26.41	2.18
Completeness [%]	99.8	99.9
Redundancy	5.1	4.8
CC(1/2)	0.996	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.4 M Ammonium Acetate, 20% PEG 2000 MME, Cryoprotectant-Ethylene glycol