6X9X
Crystal structure of Fab fragment of Anti-HCV E2 antibody (HC84.26)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.535, 113.699, 122.696 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.900 - 1.800 |
R-factor | 0.17993 |
Rwork | 0.178 |
R-free | 0.20966 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5esa |
RMSD bond length | 0.012 |
RMSD bond angle | 1.472 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.053 | 0.649 |
Rmeas | 0.059 | 0.728 |
Rpim | 0.025 | 0.326 |
Number of reflections | 53599 | 5293 |
<I/σ(I)> | 26.41 | 2.18 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 5.1 | 4.8 |
CC(1/2) | 0.996 | 0.771 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.4 M Ammonium Acetate, 20% PEG 2000 MME, Cryoprotectant-Ethylene glycol |