6X9P
2-deoxyribose-bound structure of Marinomonas primoryensis PA14 carbohydrate-binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2019-12-07 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.220, 50.530, 79.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.730 - 1.000 |
| R-factor | 0.1296 |
| Rwork | 0.129 |
| R-free | 0.14160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j6y |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.222 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.740 | 1.000 |
| High resolution limit [Å] | 0.960 | 0.960 |
| Rpim | 0.018 | 1.468 |
| Number of reflections | 106580 | 7177 |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 95.8 | |
| Redundancy | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 298 | 0.2 M calcium chloride, 0.1 M HEPES (pH 7), 20% (v/v) polyethylene glycol 3350 and 30% (w/v) 2-deoxy-ribose |
