6X8C
Self-assembly of a 3D DNA crystal lattice (4x5 junction version) containing the J1 immobile Holliday junction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 67.926, 67.926, 59.262 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.467 - 3.098 |
R-factor | 0.2416 |
Rwork | 0.238 |
R-free | 0.26710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kek |
RMSD bond length | 0.004 |
RMSD bond angle | 0.604 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.150 |
High resolution limit [Å] | 3.098 | 8.360 | 3.100 |
Rmerge | 0.065 | 0.021 | 0.342 |
Number of reflections | 3041 | 174 | 130 |
<I/σ(I)> | 12.8 | ||
Completeness [%] | 99.4 | 97.8 | 93.5 |
Redundancy | 9.8 | 9.2 | 7 |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 50 mM cacodylate pH 6.0, 20 mM MgCl2, 1.0 mM spermine, and 15% ethanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |